Journal: Bioactive Materials

Article Title: Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair

doi: 10.1016/j.bioactmat.2025.07.017

Figure Lengend Snippet: Histological evaluation of triphasic scaffold structure and matrix remodeling. FFPE sections of hMSC-seeded triphasic scaffolds (n = 2) at both Day 1 and Day 21 were stained with either trichrome, alizarin red, or alcian blue staining. Representative images are provided of (A) a scan of a full scaffold (trichrome only), (B) the osseous region, (C) the tendinous region, and (D) the interfacial region.

Article Snippet: Samples were stained by one of four staining procedures: hematoxylin/eosin (H&E, SL200 & SL201, StatLab), alizarin red (Cat# 2003999, EMD Millipore Corp.), trichrome (Cat# ab150686, Abcam), or alcian blue (pH 2.5, Cat# KTABP2.5, StatLab) with staining procedures followed according to manufacturer recommendation.

Techniques: Staining

Journal: PLoS ONE

Article Title: Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats

doi: 10.1371/journal.pone.0246363

Figure Lengend Snippet: (A) Detection of mucin in Alcian blue stained transverse colon tissue. Mucin secreted from crypt layer cells was stained with Alcian blue at pH 2.5, and images were observed at 100× magnification. Four to six rats per group were used in the preparation of tissue slide, and Alcian blue staining analysis was performed in duplicate in each slide. (B) RT-qPCR analyses for MUC2, AQP3 and AQP8. The levels of MUC2, AQP3 and AQP8 transcripts in the total mRNA of transverse colons were measured by RT-qPCR using specific primers. The mRNA levels of three genes were calculated, based on the intensity of actin as an endogenous control. Four to six rats per group were used the preparation of total RNA, RT-qPCR analyses were assayed in duplicate for each sample. The data are reported as the mean ± SD. *, p<0.05 compared to the No treated group. # , p<0.05 compared to the Lop+Vehicle treated group. Abbreviations: Lop, Loperamide; RT-qPCR, Quantitative real time-PCR; MUC2, Mucin 2; AQP, Aquaporin.

Article Snippet: The mounted tissue sections were rinsed with distilled water and stained using an Alcian Blue Stain kit (IHC WORLD LLC, Woodstock, MD, USA), and morphological features in the stained colon sections were observed by light microscopy.

Techniques: Staining, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways

doi: 10.1016/j.mcpro.2025.100962

Figure Lengend Snippet: Histological staining of extracellular matrix proteins in CAPJ-treated wound tissues. Rat skin cutaneous wound was treated with CAPJ for 60s. CAPJ-treated wound tissue section (CAPJ), with parallel untreated control (Ctrl), were prepared on day-3, -7, and -14, and subjected for histochemical staining by using Masson’s Trichrome Stain kit for collagen staining ( top panel, in dark blue color), Elastic Stain kit for elastin staining ( middle panel, in black color), and Alcian Blue Stain kit for hyaluronic acid (HA) staining ( button panel, in light-blue color). The red arrows were the extensively stained area for collagen and elastic fibers formation as well as the conjugates of HA and proteoglycans. Right panel correspond to high-magnification images of each cropped area on low-magnification image. Scale bars in low- and high-magnification images are 20 μm and 5 μm, respectively. CAPJ, cold atmospheric plasma jet.

Article Snippet: Tissue section (5 μm thick) was prepared in a standard fashion and observed in detail using Masson’s Trichrome Stain kit (StatLab, #KTMTR), Elastic Stain Kit (Modified Verhoeff's, ScyTek, ETS-1-IFU) and Alcian Blue (pH 2.5) Stain Kit (ScyTek, AFR-1-IFU).

Techniques: Staining, Control, Clinical Proteomics

Journal: Bioengineering

Article Title: Measurement of the Adipose Stem Cells Cell Sheets Transmittance

doi: 10.3390/bioengineering8070093

Figure Lengend Snippet: ( A ) Harvesting and lifting of the ASC cell sheets with a CellShifter. 1 : Cutting of the cell sheet edge, with forceps; 2 : lifting of the cell sheet using a cell shifter; 3 : transferred cell sheets in a new cell culture dish. ( B ) Dyeing of the ASC cells with Alcian blue and Alizarin Red after 2 and 3 weeks of differentiation into chondrocyte and osteoblast, respectively. (Scale bar are 200 µm).

Article Snippet: Chondrocyte cells were stained with the Alcian Blue Stain kit (Bioquochem, Llanera, Spain) and the osteoblast cells were stained with the Alizarin Red Stain kit (Millipore-Sigma, Burlington, MA, USA).

Techniques: Cell Culture